Firma:              Pharmacia

Modell:            FPLC

Kommentar:   Dokumente engl.

 

 

FPLC System for the high performance purification and characterization of proteins, peptides and nucleic acids.

 

High performance purification whatever the technique

•            Fast high capacity preparative separations for all biomolecules

•            Media and pre-packed columns for all techniques

•            Highest resolution separations with Mono Q, Mono S and Superdex

•            FPLCdirector for complete system control and reporting

•            FPLCassistant for interactive knowledge/information Support

 

FPLC is an integrated, optimized system for the high performance purification of proteins, peptides and oligonucleotides. Media and pre-packed columns for all chromatographic techniques can be used with FPLC, including Mono Q, Mono S, Superdex and our latest developments SOURCE and RESOURCE.

FPLC is fully biocompatible, ensuring recovery of biological activity. It is chemically resistant, withstanding corrosion, high density salz solutions, extremes of pH and the strong chemical agents used in high performance Systems.

FPLCdirector is the complete Software control system for programming, running, evaluating and reporting your results.

 

FPLC System (with FPLCdirector)

System Specification

Programming capacity                 ∞ individual methods

Flow rate range                           0.1-500 ml/h

Pressure range                           0-4.0 MPa up to 200 ml/h

                                                0-2.5 MPa in the range of 200-499 ml/h

Sensidvity                                  0.001-2 ,0 AU

Collection modes                        Time/Volume 0.01 -9999 min/ml

                                                Drop 1-9999 drops

Fraction collection                       up to 175 fractions

Pump control                             Controls up to 2 stepper motor pumps for gradient formation

                                                and 1 stepper motor pump for sample loading

Recorder control                         Start/stop, Chart speed, pen down/up

Valve control                               6 MV motor valves and Outports for 3 solenoid valves

Gradient formation                       Two pump gradients

Temperature range                      4-40° C

FPLC-Systems are modular designed.

Please, select the modules.

 

 

High Precision Pump P-500

Introduction

The High Precision Pump P-500 is a laboratory pump for use in liquid chromatography and other applications where constant flow at pressures up to 4 MPa (40 bar , 580 psi) is required. Pump P-500 is very accurate and reproducible, especially at low flow rates over the whole pressure range. The excellent chemical resistance of the pump makes it possible to work with corrosive liquids, e.g. organic solvents, as well as aqueous solutions.

Pump P-500 is designed to facilitate routine chromatography work. The flow rate is easily and accurately set with a three digit thumb-wheel switch. The wide flow range makes the pump suitable both for analytical and preparative chromatography. A unique wash program provides fast and secure solvent change-over and a pressure Limit control protects the column from damaging pressures.

Together with the Gradient Programmer GP-250 Plus or the Liquid chromatography Controller LCC-501 Plus, the Pump P-500 can be used to form accurate and reproducible gradients.

Pump P-500 is fully compatible with other equipment such as the single and dual path UV-monitoring Systems UV-M 11, UV-1, and their associated recorders and with the Fraction Collectors FRAG-100, FRAC-200 and SuperFrac.

Pump P-500 is designed to work with a wide range of columns and gels supplied by Amersham Biosciences/Pharmacia Biotech.

 

Specifications P-500

Flow rate range                       1-499 ml/h variable in steps of 1 ml/h.

Operating pressure                  4.0 MPa (40 bar, 580 psi) up to 200 ml/h.

                                            2.5 MPa (25 bar , 360 psi) in the range 200-499 ml/h.

Flow rate accuracy                  ±1.5% of setting or ±0.5 ml/h (which euer

Reproducibility                        Better than 0.5% set flow rate.

Flow rate precision                  Better than 1% over 8 hours at 160 ml/h wich the same solvent and temperature.

Materials of wetted parts          Fluoroplastics (Kel-F, Tefzel, Teflon), PEEK, PFE (Simriz )® , titanium and borosilicate glas.

Pressure read out                   0-5.0 MPa (0 -50 bar, 0-700 psi). Pressure recording 0.5 MPa (5 bar , 70 psi)/mV.

Pressure limit                         Protective shutdown, adjustable in the range 0-5.0 MPa (0 -50 bar, 0-700 psi).

Power supply, voltage              100/120/220-230/240 V~

frequency                               50-60 Hz

Power consumption                 32 VA

Environment                           +4 to 40 °C, 20-95% relative humidity,

                                            84-106 kPa (840-1060 mbar) atmospheric pressure.

Dimensions                            410x217x165mm.

Weight                                   9 kg.

Motor                                     Stepper motor (110 pulses/0.1 ml).

EMC standards                       This product meets the requirement of the EMC Directive 89/336/EEC through the harmonized standards EN 50081-2 (emission) and EN 50082-1 (immunity). Note: This is a class A product. In a domestic environment this product may cause radio interference in which case the user may required to take adequate actions.

Note: The declaration of confirmity is valid for the Instrument when it is:

                                            •   used in laboratory locations

                                            •   used in the same state as it was

delivered from Amersham Biosciences AB except for alteration described in the User Manual.

                                            •   used as "stand alone" unit or connected to other CE labelled Amersham Biosciences products or other products as recommended.

Safety standards                     This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the harmonized Standard EN 61010-1.

 

HiLoad Pump P-50

Introduction

The liquid chromatography HiLoad® Pump P-50 is a robust biocompatible pump for use in liquid chromatography and other applications where constant flow rates up to 49.9 ml/min at pressures up to 0.5 MPa (5 bar) are required. Pump P-50 is accurate and reproducible over a wide range of flow rates, and is suitable for long-terrn use in dernanding environments.

Pump P-50 is designed for ease of use and minimum maintenance. The flow rate is quickly and accurately set with a 3-digit thumbwheel switch. A pressure sensor stops the pump if the back pressure exceeds 0.5+0.65 MPa, preventing damage to chromatography columns.

A remote control connector allows Pump P-50 to be used with Gradient Programrner GP-250 Plus, Liquid Chromatography Controller LCC-500 Plus, LCC-50i Plus or GradiFrac. Pump P-50 is also fully compatible with other liquid chromatography equipment such as UV-monitors UV-M II, UV-1 and Uvicord® Sil and fraction collectors FRAC-100, FRAC-200 and SuperFrac®.

Pump P-50 can be used with a wide range of columns and separation media for preparative chromatography supplied by Pharrnacia Biotech.

 

Specifications P-50.

Flow rate range                        0.1+49.9 ml/min (stand-alone)

0.03+49.9   ml/min (controller)

Reproducibility                          ±2%

Flow rate adjustment                Thumbwheel: 0.1 ml/min increments

                                             Remote: 0.01 ml/min increments

Step volume                             0.4 m l

Operating pressure                   0.5 MPa (5 bar)

Pressure limit                          0.5+0.65 MPa (5+6.5 bar)

Wetted materials                      Titanium, ETFE, PMP, EPDM, Cheram (Al ₂ O ₃ ) ,PTFE , PP, PEEK

Power supply, voltage*              100/120/220-230/240 V ~

frequency                                50-60 Hz

Power consumption                  35 VA maximum

Environment                             Ambient temperature             +4 to 40 °C

                                             Relative humidity                  20-95%

Atmospheric pressure            84-106 kPa (840-1 060 mbar)

Dimensions                             270/170/290 mm

Weight                                    8 kg

EMC standards                        This product meets the requirement of the EMC Directive 89/336/EEC through the harmonized standards EN 50081-2 (emission) and      EN 50081-2 (immunity).

Note: This is a class A product. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate actions.

Note : The deciaration of conformity is valid for the

instrument when it is

•    used in laboratory locations

•    used in the same state as it was delivered from Pharmacia Biotech AB except for alteration described in the User

Manual

•    used as “stand alone “ unit or connected to other CE labelled Pharmacia Biotech products or other products as

recommended

Safety standards                      This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the harmonize standard EN 61010-1.

Liquid Chromatography Controller LCC-500 Plus

Introduction

The Liquid chromatography Controller LCC-500 PLUS is a powerful, multi-functional control unit for chromatography systems. Its programmable memory allows you to control chromatographic procedures, modify separation parameters, perform multi-dimensional chromatography, evaluate and print-out data from the chromatogram. You can also control auxiliary equipment such as fraction collectors and Chart recorders at the saure time.

Fifty independent methods can be programmed and stored in the battery backed-up memory of the LCC-500 PLUS. Each method may be accessed individually, linked to other methods or called upon as

a submethod. In addition, methods or parts of methods may be looped up to 99 times. Programmed settings may be overridden manually at any stage.

Together with prepacked columns, pumps, valves, monitors, fraction collectors and automatic injector, the LCC-500 PLUS forms fully automated systems for fast, high-resolution liquid chromatography, such as FPLC® System and BioPilo System.

 

Specification LCC-500 Plus

Method number

50 methods can be programmed individually. The methods are divided into 5 different method banks with 10 methods in each bank.

Memory capacity

750 instructions can be programmed if maximum peak memory is set to 20 (default). Otherwise every peak more than 20 will decrease the number of instructions available by 2 or 3. Minimum memory capacity is 569 instructions if 99 peaks are specified. Maximum memory capacity is 791 instructions if 2 peaks are specified.

Memory protection

The memory is backed-up with a lithium battery that will last for

a minimum of 5 years. A LED indicates when the battery should be replaced.

Display

16 digit alphanumeric display with 6 LEDs for status Information.

Keyboard

29 touch-type keys with audible tone acknowledgement. The tone will become louder when incorrect key entries are made.

Alarm

An audible alarm can be programmed to sound at any time (volume) with a duration of 0-99 min in increments of 0.1 min. There will also be a 30 s alarm when a check Code is indicated in the display.

Method base

Programmed instructions are executed in either time or volume base depending an whether the LCC-500 PLUS is calibrated in min, ml or dl; 0-9 999 min( m1,di).

Increment:

0.01 in the region 0-99.99

0.1 in the region 0-999.9

1 in the region 1 000-9 999

Sub-method call

Programmed methods can be called upon as sub-methods. Maximum is 9 levels of nesting.

Looping

A part or a whole method can be looped 1-99 times.

Gradient formation

The gradient is formed by the control of either two pumps or one pump and a switch valve. Eluents are then mixed in a dynamically stirred mixing chamber. The supplied mixer has a volume of 0.6 ml, with magnetic stirring.

Concentration

0-100 %B in 0.1 % increments.

Gradient profiles

Any gradient profile can be programmed in linear Segments of different slopes. Linear gradients are programmed by entering the time (volume) and concentration of eluent B at the breakpoints. The concentration entered will be reached at the programmed time (volume). The concentration will be changed linearly between the breakpoints.

Data collection

Signals from 2 monitors within the input range –1 500 to +1 500 mV can be collected.

Peak detection

Two monitor inputs can be used for peak detection, but only one at a time. The value of the one not used for peak detection is however stored at peak maxima.

A peak is registered when the monitor input is above the set peak threshold level (% full scale deflection, FSD). Start of peak is the set level +2 or 2 % FSID above a minimum; end of peak is the set level –2, i.e. a hysteresis of 4.

Both peak monitor values and peak threshold level are referred to the current baseline of the respective monitor. The LCC-500 PLUS stores a new baseline value when the instruction MONITOR is executed.

lf , while running a method, the monitor value is below the programmed peak threshold level and slowly changing within the programmed maximal baseline drift, the LCC-500 PLUS updates and stores the monitor value as the new baseline.

When the monitor input is above the peak threshold level, the LCC-500 PLUS does not account for baseline drift, but refers all monitor inputs

to the baseline value stored at the moment the peak threshold level was passed. Baseline drift is then not accounted for until the monitor input is below the peak threshold level again.

Baseline drift is only compensated for if the threshold level is set to a non-zero value. A filter constant >2 must be programmed when the baseline function is used (for more Information about filter constants See section 4.2 "Calibration block").

Peak memory

Data from up to 99 peaks can be stored.

Peak evaluation

The following data from up to 99 peaks can be printed out:

–           total accumulated area integrated in units of % full scale deflection (FSD) x min or % FSD x ml(dl)

–           elution time (volume) in min(m1,di) at peak maximum

–           duration in time(min) or volume(m1,dI) of a peak (peak width measured at peak threshold)

–           area of a peak in % FSD x min or % FSD x ml(di)

–           area of a peak in % of the accumulated area

–           height of the peak from both monitors at peak Max

–           event marks from the fraction collector

–           tube numbers of the peak maximums

–           runcounter value between 1 and 9 999 that is incremented for each run.

Printer/plotter

The LCC-500 PLUS can control the built-in printer/plotter for listing of methods, calibration values and peak data. The printer can be made to plot and/or print data from the chromatogram by manual activation or through instructions in a programmed method.

If mains power failure occurs, a print-out containing method number, bank number, method time (volume) and retention time (volume) will be carhed out. The run will continue when the power returns.

Printer – 20 columns, alphanumeric, 5x7 matrix.

Plotter – 140 points an Y axis.

Print speed – 1 to 1.5 lines/second.

Thermal paper – length 25 m, width 60 mm, diameter max. 47 mm. Unprinted and printed paper should be stored in darkness in a cool, dry room.

A correct printing is only guaranteed when thermal paper type JUDO TP50 KS-F is used (Code no. 19-6828-01).

Programmahle outport

Seven programmable ON/OFF outports, e.g. for control of accessory equipment, TTL compatible. Three are designed for control of a fraction collector or automatic injector. The polarity for each outport can be programmed in the calibration block.

24 V Switched power outports

Three 24 V (max. 100 MA) switched power outports are available, of which two are designed to power solenoid valves etc and one for making one-pump gradients. The third is also useable as an outport for solenoid valves when using two pumps for gradierst formation. The power outports are powered down 30 ms alter any of the outports are activated to avoid overheating the valves.

Power 24 V

A 24 V power source for external devices.

Method control

Four inports for remote method control and indications, e.g. level Sensors, TTL compatible.

Temperature range    4-40°C

Dimensions                 408x308x122 mm

Weight                        6 kg

Power requirement    60 W

Voltage                       100-120 V ±10% 220-240 V ±10%

Frequency                  50-60 Hz

Safety Standard          Electronics designed according to IEC 438 and UL 1262 requirements.

Power failure/ disturbances

In the event of power failure the System will shut down. When the

power returns the run will continue exactly where interrupted and you will get a print-out (See Printer/plotter).

Pump control

The LCC-500 PLUS can control pump functions such as RUN/PAUSE, flow rate and wach program. If the pump is inoperable or is busy, a check code is given in the display. All signals are TTL compatible.

Flow rate

0-999 ml/min in min mode

0.02-75.0 ml/min in ml mode

0.02-75.0 dl/min in dl mode

Increment.

0.01 in the region 0.00-9.99 0.1 in the region 10.0-99.9 1 in the region 100-999

The Pumps P-1, P-500, P-3500 and P-6000 can be controlled within their whole flow rate range.

Valve control

The LCC-500 PLUS can control up to 6 motor-driven valves, MV-7, PMV-7, IMV-7, MV-8, PMV-8 and IMV-8. The allowed positions can be specifically addressed. If the addressed position is not found, an alarm together with a check code is given and the LCC-500 PLUS is paused.

MV-7, PMV-7, IMV-7

The MV-7, PMV-7 and IMV-7 can be addressed to their three positions LORD, INJECT and WASH. Addressing the valves to any other position will give rise to an audible alarm and a check code will appear in the display.

MV-8, PMV-8, IMV-8

The MV-8, PMV-8 and IMV-8 can be addressed specifically to any of their 8 positions. They can also be stepped forward (clockwise) to the next position.

Recorder control

Chart speed

0-999 cm/ min( m1,dl)

Increment:

0.01 in the region 0.00-9.99

0.1 in the region 10.0-99.9

1 in the region 100-999

Pen down

Output to control a solenoid pen down function an a recorder. Automatic pen down when Chart speed is set to a non-zero value.

Gradient 1 V

Analog signal, 0-1 V, reflecting the current concentration in % of eluent B during a run. 1 V corresponds to 100 %B. Resolution 0.001 V (0.1 %B).

Fraction collector control

The LCC-500 PLUS can control a fraction collector to RUN/STOP, PAUSE/CONTINUE, FEED TUBE and RECYCLE (recycle is possible only with FRAC-200 and FRAC-300 R).

Liquid Chromatography Controller LCC-501 Plus

Introduction

The Liquid chromatography Controller LCC-501 Plus is a powerful, multi-functional control unit for chromatography systems. Its programmable memory allows you to control chromatographic procedures, modify separation parameters, perform multi-dimensional chromatography, evaluate and print-out data from the chromatogram. You can also control auxiliary equipment such as fraction collectors and Chart recorders at the saure time.

Fifty independent methods can be programmed and stored in the battery backed-up memory of the LCC-501 Plus. Each method may be accessed individually, linked to other methods or called upon as a submethod. In addition, methods or parts of methods may be looped up to 99 times.

Programmed settings may be overridden manually at any stage.

Together with prepacked columns, pumps, valves, monitors, and fraction collectors, the LCC-501 Plus forms fully automated systems for fast, high­resolution liquid chromatography, such as FPLC System and Biopilot System.

 

Specifications LCC-501 Plus

Method number

50 methods can be programmed individually. The methods are divided into 5 different method banks with 10 methods in each bank.

Memory capacity

958 instructions can be programmed if maximum peak memory is set to 20 (default). Otherwise every peak more than 20 will decrease the number of instructions available by 2 or 3. Minimum memory capacity is 777 instructions if 99 peaks are specified. Maximum memory capacity is 999 instructions if 2 peaks are specified.

Memory protection

The memory is backed-up with a lithium battery that will last for a minimum of 5 years. A Light Emitting Diode (LED) indicates when the battery should be replaced.

Display

16 digit alphanumeric display with 6 LEDs for status information.

Keyboard

29 click type keys. There will be a audible tone when incorrect key entries

are made.

Alarm

An audible alarm can be programmed to Sound at any time (volume) with a duration of 0-99 min in increments of 0.1 min. There will also be a 30 sec alarm when a check code is indicated in the display.

Method base

Programmed instructions are executed in either time or volume base depending an whether the LCC-501 Plus is calibrated in min, ml or dl; 0-9 999 min( ml, dl).

Increment:

0.01 in the region 0-99.99

0.1 in the region 0-999.9

1 in the region 1 000-9 999

Preprogrammed methods

The LCC-501 Plus has 9 different preprogrammed methods. The methods are within the Software and are copied into method bank 5 in method 0-8 by using the instruction CLEAR MEMORY 501 in the calibration block. If there already exists a method in one of these, this method will not be copied and check code 23 will be shown. There is one method for each technique (gel filtration, anion exchange, cation exchange, affinity, hydrophobic interaction and reversed phase chromatography) and three different equilibration methods.

Sub-method call

Programmed methods can be called upon as submethods. Maximum is 9 levels of nesting.

Looping

A part or a whole method can be looped 1-99 times.

Gradient formation

The gradient is formed by the control of either two pumps or one pump and a switch valve. Eluents are then mixed in a dynamically stirred mixing chamber. The supplied mixer has a volume of 0.6 ml, with magnetic stirring.

Concentration

0-100 %B in 0.1 % increments.

Gradient profiles

Any gradient profile can be programmed in linear Segments of different slopes. Linear gradients are programmed by entering the time (volume) and concentration of eluent B at the breakpoints. The concentration entered will be reached at the programmed time (volume). The concentration will be changed linearly between the breakpoints.

Data collection

Signals from 2 monitors within the input Tange —1 500 to +1 500 mV can be collected.

Peak detection

Two monitor Inputs can be used for peak detection, but only one at a time. The value of the one not used for peak detection is however stored at peak maxima.

A peak is registered when the monitor input is above the set peak threshold level (% full scale deflection, FSD). Start of peak is the set level +2 % or 2 % FSD above a minimum; end of peak is the set level —2 %, i.e. a hysteresis of 4 %.

Both peak monitor values and peak threshold level are referred to the current baseline of the respective monitor. The LCC-501 Plus stores a new baseline value when the instruction MONITOR is executed.

If, while running a method, the monitor value is below the programmed peak threshold level and slowly changing within the programmed maximal baseline drift, the LCC-501 Plus updates and stores the monitor value as the new baseline.

When the monitor input is above the peak threshold level, the LCC-501 Plus does not account for baseline drift, but refers all monitor Inputs to the baseline value stored at the moment the peak threshold level was passed. Baseline drift is then not accounted for until the monitor input is below the peak threshold level again.

Baseline drift is only compensated for if the threshold level is set to a non­zero value. A filter constant >2 raust be programmed when the baseline Fundion is used.

Peak memory   Data from up to 99 peaks can be stored.

Peak evaluation

The following data from up to 99 peaks can be printed out:

-            total accumulated area integrated in units of % Full scale deflection (FSD) x min or % FSD x ml(d1)

-            elution time (volume) in min(m1,dI) at peak maximum

-            duration in time(min) or volume(m1,dI) of a peak (peak width measured at peak threshold)

-            area of a peak in % FSD x min or % FSD x ml(d1)

-            area of a peak in % of the accumulated area

height of the peak from both monitors at peak maximum

-            tube numbers of the peak maximums

-            runcounter value between 1 and 9 999 that is incremented for each tun.

Printer/plotter

The LCC-501 Plus can control the built-in printer/plotter for listing of methods, Calibration values and peak data. The printer can be made to plot and/or print data from the chromatogram by manual activation or through instructions in a programmed method.

If mains power failure occurs, a print-out containing method number, bank number, method time (volume) and retention time (volume) will be carried out. The tun will continue when the power returns.

Printer – 20 columns, alphanumeric, 10 x 14 matrix.

Plotter – 320 points an Y axis.

Prinz Speed - 1.5 lines/second .

Thermal paper- length 25 m, width 60 mm, diameter max. 47 mm. Unprinted and printed paper should be stored in darkness in a cool, dry room.

A correct printing is only guaranteed when thermal paper type JUJO TF50 K–E is used.

Programmable outports

Seven programmable ON/OFF outports, e.g. for control of accessory equipment, TTL compatible. Three are designed for control of a fraction collector or automatic injector. The polarity for each outport can be programmed in the Calibration block.

24 V Switched power outports

Three 24 V (max. 100 mA) switched power outports are available, of

which two are designed to power solenoid valves etc. and one for making one-pump gradients. The third is also useable as an outport for solenoid valves when using two pumps for gradient formation. The power outports are powered down 30 ursec after any of the outports are activated to avoid overheating the valves.

Power 24 V       A 24 V power source for external devices.

Method control

Four inports for remote method control and indieations, e.g. level Sensors; TTL.compatible.

Temperature range    4-40°C

Dimensions                 408 x 308 x 122 mm

Weight                        6 kg

Power requirement    60 W

Voltage                       100-120 V ±10 %

                                  220-240 V ±10 %

Frequency                  50/60 ±2 Hz

Safety standard          Electronics designed according to IEC 1010 and UL 1262 requirements.

Power failure/disturbances

In the event of power failure the System will shut down. When the power returns the tun will continue exactly where interrupted and you will get a print-out (See Printer/plotter).

Pump control

The LCC-501 Plus can control pump functions such as RUN/PAUSE, flow rate and wash program. If the pump is inoperable or is busy, a check code is given in the display. All Signals are TTL compatible.

Flow rate

0-999 ml/min in min mode

0.02-75.0 ml/min in ml mode

0.02-75.0 dl/min in dl mode

Increment:

0.01 in the region 0.00-9.99

  0.1 in the region 10.0-99.9

1 in the region 100-999

The Pumps Pump P-1, HiLoadTm Pump P-50, High Precision Pump P-500 and Pump P-6000 can be controlled within their whole flow rate range.

Valve control

The LCC-501 Plus can control up to 6 motor-driven valves, MV-7, PMV-7, IMV-7, MV-8, PMV-8 and IMV-8. The allowed positions can be specifically addressed. If the addressed position is not found, an alarm together wich a check code is given and the LCC-501 Plus is paused.

MV-7, PMV-7, IMV-7

The MV-7, PMV-7 and IMV-7 can be addressed to their three positions LORD, INJECT and WASH. Addressing the valves to any other position will give rise to an audible alarm and a check code will appear in the display.

MV-8, PMV-8, IMV-8

The MV-8, PMV-8 and IMV-8 can be addressed specifically to any of their 8 positions. They can also be stepped forward (clockwise) to the next position.

Recorder control

Chart Speed     0-999 cm/ min( ml, dl)

Increment :

0.01 in the region 0.00-9.99

0.1 in the region 10.0-99.9

1 in the region 100-999

Pen down

Output to control a solenoid pen down function an a recorder. Automatic pen down when Chart speed is set to a non-zero value.

Gradient 1 V

Analog Signal, 0-1 V, reflecting the current concentration in % of eluent B during a run. 1 V corresponds to 100 %B. Resolution 0.001 V (0.1 %B).

Fraction collector control

The LCC-501 Plus can control a fraction collector to RUN/STOP, PAUSE/ CONTINUE, FEED TUBE and RECYCLE (recycle is possible only wich FRAC-200 and SuperFrac).

Gradient Programmer GP-250 Plus

Introduction

The Gradient Programmer GP-250 Plus is a powerful, multi-functional control unit for chromatography systems. Its programmable memory allows you to control chromatographic procedures, rnodify separation parameters and control auxiliary equipment such as fraction collectors and chart recorders at the same time.

Fifty independent methods can be programmed and stored in the battery backed-up memory of the GP-250 Plus. Each method may be accessed individual !y , linked to other methods or called upon as a subrnethod. In addition, methods or parts of methods may be looped up to 99 times. Programmed settings may be overridden manually at any stage.

Together with prepacked columns, pumps, valves, monitors, fraction collectors and recorders, the GP-250 Plus forms a systems for fast, high­resolution liquid chromatography, such as FPLC® System.

 

Specifications GP-250 Plus

Method number           50 methods can be programmed individually. The methods are divided into 5

                                    different method banks with 10 methods in each bank.

 

Memory capacity          Maximum memory capacity is 750 instructions.

 

Memory protection       The memory is backed-up with a lithium battery that will last for a minimum of

                                    5 years. A LED indicates when the battery should be replaced.

 

Display                          16 digit alphanumeric display with 5 LEDs for Status information.

 

Keyboard                      29 click-type keys with audible tone acknowledgement. The tone will

                                    become louder when incorrect key entries are made.

Alarm                            An audible alarm can be programmed to sound at any time (volume) with a

                                    duration of 0-99 min in increments of 0.1 min. There will also be a 30 s alarm

                                    when a check code is indicated in the display.

 

Method base                 Programmed instructions are executed in either time or volume base depending on

                                    whether the GP-250 Plus is calibrated in min, ml or dl; 0-9 999 min(ml,dl).

 

Increment:                      0.01 in the region 0-99.99

                                    0.1 in the region 0-999.9

                                    1 in the region 1 000-9 999

 

Sub-method call           Programmed methods can be called upon as sub-methods. Maximum is of nesting.

 

Looping                        A part or a whole method can be looped 1-99 times.

 

Gradient formation                The gradient is formed by the control of either two pumps or one pump and a switch valve. Eluents are then mixed in a dynamically stirred mixing chamber. The supplied mixer has a volume of 0.6 ml, with magnetic stirring.

 

Concentration                          0-100% B in 0.1% increments.

 

Gradient profiles                     Any gradient profile can be programmed in linear segments of different siopes. Linear gradients are programmed by entering the time (volume) and concentration of eluent B at the breakpoints. The concentration entered will be reached at the programmed time (volume). The concentration will be changed linearly between the breakpoints.

 

Programmable outports          Seven programmable ON/OFF outports, e.g. for control of accessory equipment, TTL compatible. Three are designed for control of a fraction collector or automatic injector. The polarity for each outport can be programmed in the Calibration block.

 

24 V Switched power             Three 24 V (max. 100 MA) switched power outports are available, of which

outports                                 two are designed to power solenoid valves etc. and one for making one­pump gradients. The third is also useable as an outport for solenoid valves when using two pumps for gradient formation. The power outports are powered down 30 ms after any of the outports are activated to avoid overheating the valves.

 

Power 24 V                             A 24 V power source for external devices.

Memory protection                  Four inports for remote method control and indications, e.g. level sensors,

                                               TTL compatible.

Environment                            +4 to +40 °C, 20-95% relative humidity,

                                              84-106 kPa (840-1060 rnbar) atmospheric pressure

                Dimensions              408x308x122 mm

Weight                                    5.5 kg

Power requirement                50 VA

 

Voltage                                    100/120/220-230/240 V

Always connect the instrument to a properly grounded mains outlet.

 

Frequency                               50-60 ± 2 Hz

EMC standards                       This product meets the requirement of the EMC Directive 89/336/EEC through the

                                                             harmonized standards EN 50081-2 (emission) and EN 50082-1 (immunity)

 

Note:                This is a class A product. In a domestic environment this product may cause radio                        interference in which cases the user rnay be required to take adequate actions.

 

Note:            The deciaration of conforrnity is valid for the instrument when it is

 

•    used in laboratory locations

•    used in the same state as it was del ivered from Pharmacia Biotech AB except for alteration described in the User Manual

•    used as “stand alone “ unit or connected to other CE labelled Pharmacia Biotech products or other products as recornmended

Safety standard                       This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the harmonized standard EN 61010-1.

Power failure/                         In the event of power failure the system will shut down. When the disturbances    disturbances power returns the run will continue exactly where

                                               interrupted .

 

Pump control

The GP-250 Plus can control pump functions such as RUN/PAUSE, flow rate and wash program. If the pump is inoperable or is busy, a check code is given in the display. All signals are TTL compatible.

 

Flow rate                                 0-999 ml/min in min mode

0.02-75.0    ml/min in ml mode

0.02-75.0    dI /min in dl mode

Increment:

0.01 in the region 0.00-9.99

0.1    in the region 10.0-99.9

1 in the region 100-999

The Pumps P-1, HiLoad ^TM Pump P-50, High Precision Pump P-500, and P-6000 can be controlled within their whole flow rate range.

 

 

        Recorder control

Chart speed                             0-999 cm/ min( ml,dl)

                                            Increment:

  0.01           in the region 0.00-9.99

  0.1            in the region 10.0-99.9

Pen down                              Output to control a solenoid pen down function on a recorder. Automatic pen down when chart speed is set to a non-zero value.

Gradient 1 V                           Analog signal, 0-1 V, reflecting the current concentration in % of eluent B during a run. 1 V corresponds to 100% B. Resolution 0.001 V (0.1% B).

Fraction collector control

The GP-250 Plus can control a fraction collector to RUN/STOP, PAUSE/CONTINUE, FEED TUBE and RECYCLE (recycle is possible only with FRAC-200 and SuperFrac ^TM ).

Fraction Collector FRAC-100 and FRAC-200

Introduction

The Pharmacia LKB fraction collectors, FRAC- 100   and FRAC-200 , are versatile Instruments for automated fraction collection and evaluation of chromatograms. In their standard forms they can collect up to 1.75 fractions in 12 mm diameter tubes, up to 95 fractions in

10-18 mm diameter tubes and up to 45 fractions in 28 mm diameter vials, with the help of the racks provided or with simple accessories. When connected to a suitable monitor, they can measure peak heights, areas and retention for up to 11 peaks (FRAC-100) or 19 peaks (FRAC-200). The fraction collectors will also indicate into which tubes the peaks haue been collected.

FRAC-200 and FRAC-100 (with its drop counting accessory) can collect fractions in a variety of different ways, based either an time, volume or drop counting, controlled via simple programs specified by the Operator. For example, you can choose to collect uniform fractions of a fixed time period. The fraction collectors will collect fractions until all the tubes are used and then switch off. In other collection sequences, you can specify a "wait" period in which the initial liquid volume is collected in a separate container, collect peaks in a smaller fraction size than the main pari of the eluent, collect only peaks and allow the remainder to run into a separate container, compensate for the liquid volume bet-ween the monitor and the fraction collector and program a time, volume or drop count for the collector to shut itself, and a connected pump, off automatically. At any time during collection you can advance to a new tube or stop the collection temporarily before continuing with the rest of the program.

These fraction collectors are designed to work with other equipment from Pharmacia LKB, including Peristaltic Pump P-1, Pump P-50, chromatography Controllers, Chart recorders and UV-monitors to form integrated chromatographic Systems adapted to your present and future requirements.

 

Specifications FRAC100 and FRAC200

Tube                     Diameter               Height                     Max. number

racks                     range                    range                      per rack

12 mm rack            12 mm                   50-180 mm               175

18 mm rack            10-18 mm               50-180 mm*              95

28 mm rack            28 mm                   30-180 mm               45
lf >100 mm, diameter must be 10% of height

Tubing                              1.7-2 mm outer diameter

Tube change time             Maximum tube change times for each Crack

                                      ( outer to inner Cracks)

Tube rack 12 mm              Track 1           0.2 sec Track 3 0.3 sec

                                      Track 5           0.4 sec

Tube rack 1.8 mm             Track 1           0.3 sec Track 2 0.4 sec

                                      Track 3           0.5 sec Track 4 0.6 sec

Tube rack 28 mm              Track 1           0.4 sec Track 2 0.55 sec

                                      Track 3           0.7 sec

Fraction size

Time                                0.01-99.99 min (0.01 min increments)

                                      100-999.9 min (0.1 min increments)

1000-9 999 min (1 min increments) within an accuracy of ± 0.02%

Volume                            0.01-99.99 ml (0.01 ml increments)

                                      100-999.9 ml (0.1 ml increments)

1 000-9 999 ml (1 ml increments) within and accuracy of ± 0.02%

Drops                               1-9 999 (1 drop increments) FRAC-100 requires optional accessory

Max drop rate                   approximately 15 drops/s

Peak memory                   Stores up to 11 peaks (FRAG-100), or 19 peaks (FRAG-200)

Peak evaluation                Displays peak height (% Full Scale Deflection, FSD), peak area (min% FSD or ml% FSD or drop% FSD); elution time (min), volume (m1) or drop number at peak maximum; duration in time, volume or drops of peak; tube number of first and last tubes containing peak and tube at peak maximum

Run evaluation                 Displays time, volume or drop number remaining in current fraction; tube number, time, volume or drop number from starr; flow rate (m1, drops/min only), monitor Signal (% FSD) total number of

                                      peaks collected; time, volume or drop number of last power failure

Memory protection            Programmed parameters and stored data are guaranteed for at least 1 hour after the Instrument is switched off or in the event of a power failure. Under normal conditions at least 4 4 days storage can be expected

Event mark                       0.2 s for both contact closure and TTL outport in remote Bocket (see Section 3.6). Programmahle delay (see Section 4.3)

Chemical resistance          The bowl and the racks are made of PBT (Valox) which is generally r     esistant to:

                                      •         weck bases: e.g. diluted solutions of ammonium, amines etc.

                                      •         alcohols : e.g. methyl-, ethyl-, propylalcohol etc. and some glycols.

PBT is also resistant to cleaning detergents and chemicals, hot water, UV-radiation.

Avoid contact the following groups of chemicals; should spillage occur clean immediately:

                                      •         Oxidizing components, e.g. nitric acid peroxides.

                                      •         Strong bases, e. g. sodium or other metal hydroxides, some amines.

                                      •         Chlorinated solvents, e. g. methylene chloride, ethylene chloride, trichlorethane.

                                      •         Esters e. g. acetates.

                                      •         Aromatic hydrocarbons, e. g. ketones and aldehydes.

Remote control               15-pin female D-type connector, TTL compaüble
Signals (see Section 3.6)

                                      Inport:             Gutport:

                                      recycle (FRAG-200)     event mark

                                      flow rate          stop pump

                                      run      FRAG operable pause

                                      feed

                                      remote Controller operable

Printer Output                   25-pin female D-type connector; RS 232 C serial Interface; Baud rate 9600 wich 8 biss and 1 stop bit. FRAC-200 only

Valve output                     Nominal 24 V output, screw contacts

Monitor Input                    10, 100 or 1000 mV; 5 readings/s; screw contacts

Voltalte                            100/120 /220/240 ± 10% VAC

Frequency                        48-62 Hz

Power consumption           15 W

Operating temperature 4-40 °C

Dimensions                      290x300x390 mm (WxDxH)

Weight                             5.5 kg (12 lb)

Safety Standards              Electronics designed according to IEC and UL requirements

Fraction Collector Super Frac

5

Introduction

Fraction Collector SuperFrac ^TM , is a sophisticated, high-capacity

preparative instrument enabling a wide variety of users to collect fractions or peaks according to their exact requirements.

To simplify operation an integrated microprocessor helps the user through the basic programming sequence. Optimal functions and parameters can be programmed independently.

SuperFrac fraction collector offers true peak-siope collection, time window collection, and many other advanced features. Peak-slope collection and time window collection are especially useful for repetitive preparative runs in lab scale, and pilot scale, where the ability of SuperFrac to accept many very large tubes is indispensable.

Numerous combinations of collection modes make SuperFrac the most flexible fraction collector available. When remotely controlled from the LCC-501 Plus, GP-250 Plus or LC Controller 2252, SuperFrac will perform repetitive collection in loops. This feature is useful for large-scale, FPLC® and HPLC preparative runs. SuperFrac will store all parameters for nine complete programs.

The SuperFrac is also designed to work with other equipment from Pharmacia Biotech such as peristaltic pumps, UV-monitors and recorders to form integrated chromatographic systems adapted to your requirements.

 

Technical Specifications SuperFrac

Tube capacity                             Rack A: 312 tubes, 12-13 mm

                                                Rack B: 180 tubes or scintillation vials, 16-18 mm

                                                Rack C: 84 scintillation vials, 28 mm

                                                Rack D: 120 scintillation vials, 16-18 mm

                                                Rack E: 60 scintillation vials, 28 mm

 

 

Collection modes

Time collection                           1 s to 99 min 59 s in 1 s intervals or

                                                1 min to 99 h 59 min in 1 min intervals

Volume collection                       0.01 ml to 10 1 per fraction

Drop collection                           1 to 9 999 drops in 1 drop intervals

Max drop rate                             12 drops/second

Step delay                                  1 s to 99 min 59 s (time mode)

                                                1-9 999 drops (drop mode)

                                                0.01 ml to 10 1 (volume mode)

Tube change time                       Rack A : 250 ms

                                                Rack B: 400 ms

                                                Rack C: 600 ms

                                                Rack D: 440 ms

                                                Rack E: 660 ms

                                                Rack F: 20 ms/mm. Movement in x :y

                                                direction

Wait time                                   1 s to 99 h 59 s

Automatic shut-down                   Auxiliary equipment can be shut-down

                                                after preset number of tubes, after end

                                                time , or after end of equilibration time

Event mark                                 Open collector output for 1 s ± 10 %

Time windows                             Nine , each with own programmable

                                                start and end time: range 1 to 99:59

                                                hour: min or min:s.

Program memory                        Stores nine complete programs

Memory back-up                         Li-battery

End time                                    1 min to 99 h 59 min

Equilibration time                        1 min to 99 h 59 mm, activated by end

                                                time

Siope detection                          Defined by threshold (1 to 999 % o FSD)

                                                and peak width at half height (1 s to

                                                99 min 59 s)

Asymmetry correction                 Adjustable between 0.1 and 99.9,

                                                defined as one tenth of the peak height

 

Isocratic peak­

broadening correction                  Adjustable between 0.00 and 99.5 9 h/

                                                min .

 

External control                          Communication with LCC-501 Plus,

                                                GP-250 Plus and LC Controller 2252:

                                                Run, Return, Pause, Feed, Start loop,

                                                New loop and Return loop.

 

Flow Wing Control                      High volume flow meter input

Environment                               +4 to +40 °C, 20-95% relative

                                                humidity , 84-106 kPa (840-1060 mbar)

                                                atmospheric pressure

 

Main voltage                               100/115/120/220—230/240 V­ ~

Requirements                             50-60 Hz

 

Power consumption                    Max 45 W when all auxiliary

                                                equipment is connected

Dimensions (WxDxH)                 550x330x430 mm

Net weight                                 15.5 kg

Gross weight                                         23 kg

REC 101 single-channel recorder and

REC 102 dual-channel recorder

Introduction

Pharmacia Biotech model REC 101 (Code No. 18-1001-42), single-channel recorder and model REC 102 (Code No. 18-1001-43), dual-channel recorder are compact recorders designed to meet the challenging demands of today‘s technology.

These Instruments are part of the new generation of high quality, flat-bed recorders from Pharmacia Biotech which use a null balancing potentiometric system. They can accommodate a range of input voltages, offer a selection of chart speeds, and allow many functions to be remotely controlled.

The recorders have sophisticated features such as electric pen lift , grid and home functions, forward and reversed paper feed. Paper is transported at a constant velocity selected from the 14 different chart speeds in the range from 0.1 mm/min to 20 mm/s, and can be synchronized with the pump to enable chromatogram comparision.

The positions of the two independent pens (REC 102) represent the leveis of the two corresponding inputs from which the time functions are drawn.

Owing to the nature of the dual-channel recorder, the pen of channel 1 is always 2.5 mm behind the pen of channel 2. Full sensitivity ranges from 1 mV to 20 V DC.

Recorder (REC 102) is equipped with one chart drive control panel and two independent, isolated servo systems with a control panel for each channel.

Recorders REC 101 and REC 102 have an attractive design with much attention having been paid to ergonomics: control functions are weil positioned, keys and switches are very accessible, and paper is easily changed. Pens, paper and keyboard are all protected from accidental spillage by the moulded cover which fits over the recorder. The pens, which are non­smear and disposable, give clear, accurate traces for instance evaluation. Chart paper is of a high quality, and 200 mm wide.

Improved control capabilities permit the use of these recorders in all Pharmacia Biotech chromatography systems.

 

Technical Specifications REC101 and REC102

 

Recording channels:              REC 101: 1-channel

                                          REC 102: 2-channels

Input ranges:                         1, 2, 5, 10, 20, 50, 100, 200, 500 mV and 1, 2, 5, 10, 20 V

                                          FSD (full scale deflection) for each channel

Variable ranges:                    40-100 % FSD

Accuracy of span:                  <0.3 % FSD

Non-linearity:                         <0.3 % FSD

Dead zone:                           <0.2 % FSD

Pen response time:                0.2 s

lnput impedance:                   1 M W DC

lnput :                                    Floating asymetrical

Input terminals:                      “Low profile “ banana plugs

input current:                         <5 nA

Maximum input voltage:          Max. 60 V between channels and max. 30 V between

                                          channel and ground

Common mode rejection:    > 130 dB

Zero adjustment range:           -50 % to +150 % of FSD

Zero position:                        Choice of left or right-hand side of chart by a switch on rear panel. (Right-hand zero point on detivery)

Zero drift:                              <1 m V/ °C

Pen lift:                                 Electrical, independent control of pen 1 and pen 2,

                                          automatic pen lift at power down or after 30 s stop of chart speed

Chart speed range:                0.1, 0.2, 0.5, 1, 2, 5, 10, 20 mm/s or mm/min

Chart paper:                          25 m roll according to DIN 16 230

Chart paper life:                     Max. 4 167 hours at lowest chart speed. Min. 20 min at

                                          highest chart speed

Pens:                                   Disposable, fibre-tip cartridge

 

 

Conductivity Monitor

 

Introduction

The Pharmacia Conductivity Monitor, is a high quality, high precision on-line monitor for conductivity measurements and gradient determination in liquid chromatography applications.

The monitor consists of two units, the flow ccli and the control unit. The flow ccli contains the titanium electrodes used for conductivity measurement as weil as a temperature sensor. The small size of the flow ceil allows it to be positioned anywhere in the liquid path, provided that the pressure specification for the cell is not exceeded.

The control unit carries the six programming keys for the monitor. An easy to read 32 digit aiphanumeric display shows the conductivity measured in Siemens/cm (mS/cm or m S/cm) and the temperature. The display also shows the relative gradient Position as a percentage of the maximum set value, as both a percentage figure and graphically.

The monitor has a very large dynamic range from 1 m S/cm to 999.9 mS/cm and is therefore suitable for a wide range of applications, ranging from reversed phase to hydrophobic interaction chromatography. A unique design of the electronics is the microprocessor control of frequency variations within the working range. This gives excellent linearity and elirninates the need for range settings.

 

Specifications Conductivity Monitor

Operating mode                         Auto range conductivity in mS/cm or m S/cm

Sensitivity range                         1 m S/cm - 999.9 mS/cm

Accuracy                                   ±2 % full scale calibrated range or ±5 m S/cm whichever

                                               is greater in the range 1 m S/cm - 300 mS/cm

Noise                                        ±0.5 % full scale calibrated range

Flow rate range                          0-100 ml/m

Analogue output                         0-1 V

Power supply                             100-240 V

Environment                               +4 to +40°C, 20-95% relative humidity,

                                               84-106 kPa (840-1060 mbar) atmospheric pressure

Power consumption                    5 W

Dimensions (w x d x h)               245 x 150 x 100 mm

Weight                                      2.3 kg

EMC standards                          This product meets the requirement of the EMC

                                               Directive 89/336/EEC through the harmonized

                                               standards EN 50081-2 (emission) and EN 50082-1

                                               ( immunity )

                                               Note: This is a class A product. In a domestic

                                               environment this product may cause radio interference

                                               in which case the user may be required to take

                                               adequate actions.

                                               Note: The declaration of conformity is valid tor the

                                               instrument when it is:

                                               • used in laboratory locations

                                               • used in the same state as it was delivered from

                                                 Pharmacia Biotech AB except for alteration

                                                 described in the User Manual

                                               • used as “stand alone “ unit or connected to other

                                               CE Iabelled Pharmacia Biotech products or other

                                               products as recommended

 

Safety standards                        This product meets the requirement of the Low Voltage

                                               Directive (LVD) 73/23/EEC through the harmonized

                                                           standard EN 61 01 0-1.

Flow cell

 

Type                                         Flow through

Cell constant                             50 cm ¹ , nominal

Internal volume                           14 m 1

Maximum operating pressure      5 MPa, 50 bar, 750 psi

Cable Iength                              1.5 m

Wetted parts                              Fluoroplastics, titanium

Fittings                                      M-6 (6 mm metric)

 

Monitor UV-M II

Introduction

The Pharmacia Monitor UV-M II , Hg Optics with 254 and 280 nm filters, Code No.18-0604-02 or Zn Optics with 214 nm filter, Code No. 18-0605-02) is a fixed wavelength monitor for liquid chromatography. The UV-M II consists of a control module and a small optical unit which makes it easy to place it optimally in the system. State of the art electronics and high precision optics offer fiexibility and accuracy in UV-M II detection. The UV-M II is ideal for all types of liquid chromatography, primarily FPLC® and HPLC.

The Optical Unit has interchangeable mercury and zinc lamps, an analytical, high precision double cone (5 mm path length) or a preparative, straight (2 mm path length) flow cell. The double cone shape of the analytical cell minimizes the collisions between light beams and cell wall and maximizes the illuminated volume, i.e. it gives an increased sensitivity. Easy selection of different filters means there is good choice for diverse application needs. This unit may be connected directly to any chromatography column, thus minimizing the dead volume between the column and the monitor.

The Control Unit has touch panel design and solid state electronics. Functions such as autozero and event mark can be executed from a remote source.

 

Technical Specifications Monitor UV-M II

Control Unit

Full scale ranges (AU)                               0.001, 0.002, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 ,2

            Autozero                                      Range                        -0.2 to 2 AU

                                                              Reproducibility            1% full scaleSet time

                                                                                              3 secondsControl

                                                                                              front switch or remote

            Base line adjust                            Adjustable up to ± 100% full scale

            Event mark                                   Will give a ± 10% of full scale spike. Control activated   by

                                                              front switch or remote

            Electronic filter                              Response time* --selectable 0.1, 1 or 10 s 10% to 90% of

                                                              full scale. 2nd degree Bessel filter for no peak distortion.

                                                              *Note : Traditionally, time constant refers to the

                                                              time taken for the output signal to rise from 0 to 63%.   Thus,

                                                              a response time here of 1.0 second corresponds to a

                                                              traditional time constant of 0.5 seconds, and so on

            Remotelnput/Output                      Connector type: 15 pin D-SUB connector (female)

                                                              Description                                                       Pin

                                                              1 V/AU (Output, not filtered)                                  11

                                                              Vr* (Output, not filtered)                                          9

                                                              Vs** (Output, not filtered)                                        2

                                                              Analog ground 10

                                                              Autozero base line (TTL input )* **                            14

                                                              Event mark (TTL input )* **                                        6

                                                              Autozero ready (TTL output )* **                                7

                                                              Digital ground                                                       15

                                                              Protectedground                                                     1

                                                              *vrvoltage on reference diode

                                                              **vs=voltage on sample diode

                                                              ***active low

Recorder output                                        100 mV full scale, over-range to 5V (50x )10 mV full scale,

    over-range to 0.5 V (50x)

                                                              0 common

                                                              c safety ground

    Connector type: screw terminal

Linearity                                                      1% up to 2 AU at 254 nm5% up to 2 AU at other wavelengths

Output impedance                   200 ohm

Environment                            +4 to +40 °C, 10—95% relative humidity,

    84—106 kPa (840—1060 mbar) atmospheric pressure

Humidity                                                     10 - 95%

            Power requirements                       100/120/220—230/240V ± 10%, 50/60 Hz

            Power consumption                       25 VA

Dimensions                             245x304x92 mm (WxLxH)

Weight                                                            2.8 kg

 

Optical Unit

Hg lamp                           254, 313, 365, 405, 436 and 546 nm:

                                      Lifetime 8 000 h.

                                      280 nm: Lifetime 4 000 h

Zn lamp                           Wavelength 214 nm: Lifetime 2 000 h

Filters                              Interference. Circular 1.27 cm diameter.

                                      Wavelengths 214, 254, 280, 313, 365, 405, 436

                                      and 546 nm

Safety standard                Electronics designed according to IEC and UL

requirements

 

                                      FIow cell 2 mm Flow ceIl 5 mm

Optical path length            2.0 ± 0.2 mm              5.0 ± 0.1 mm

llluminated volume            2 m 1                           6 m 1

Total dead volume             30 m 1                         10 m 1

Cell shape                        Straight flow                Horizontal double

                                      through cuvette           cone with inlet and outlet distributor

Cell material in direct        Quartz                        Quartz

contact with liquid             (Suprasil Il®),              (Suprasil Il®),

                                      ETFE                         titanium, ETFE

Flow range                       0-1 000 ml/h               0-1000 ml/h

Pressure                          Max 40 bar                 Max 40 bar

Tubing connection typeFlanged (FPLC)               Unflanged type (standard)

                                      Capillary 1/16 “ o.d . metal (HPLC)

Dimensions                      100x100x50 mm         (WxLxH)

Weight                             0.4 kg

 

Sensitivity specifications

Static short term noise             ±3x10 ^-6 AU

Dynamic short term noise         ±6x10 ^-6 AU

Static long term noise               ±2x10 ^-5 AU

Dynamic long term noise          ±4x10 ^-5 AU

Static drift                                ±1x10 ^-4 AU/h

Dynamic drift                           ±2x10 ^-4 AU/h

Flow sensitivity                        2x10 ^-4 AU min/mI

 

Test conditions

Dynamic condition            1 ml/min methanol

Response time                 1 s

Room temperature            25±0.2 °C

Wavelength                      254 nm

Flow cell                          5 mm cell

 

Uvicord SII

Introduction

Pharmacia Biotech Uvicord SII Code No. 18-1004-50 is a Compact single beam UV-monitor with flow-through cell for monitoring the eluant from various column Separations. Available wavelengths, selected by a combination of lamp and interference filier are 206, 226, 254, 280, 313, 365 and 405 nm.

Pharmacia Biotech Uvicord SII is housed up in one combined optical and electronic unit. This detector simultaneously produces two absorbance Outputs - one fixed at 2 AUFS and one selectable from 0.005 - 2 AUFS in 9 steps. Thus, with the selection of the appropriate absorbance Tange, Uvicord SII enables monitoring of both high and low concentration substances simultaneously with a dual-channel recorden

Substances such as non-aromatic peptides, amino acids, lipids, nucleotides and certain carbohydrates haue strong absorption at 206 nm whereas at higher wavelengths they Show little or no absorption. Furthermore, most proteins also exhibit greater absorption at 206 nm than they do at 280 nm. By employing all fully integrated electronics and the very Tatest detection principles Uvicord SII performs-- to specification at ambient temperature and under cold room conditions.

 

Specifications Uvicord SII

Operating wavelengths             206, 226, 254, 280, 313, 365 and 405 nin .

Absorbance ranges                 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 and 2 AUFS.

Noise                                     <lx10-4 AU measured peak to peak in a dry cell.

Drift                                       <5x10-4 AU/h at constant temperature.

Operating temperature             0 to +30°C.

Recorder Outputs                    100 mV for selected absorbance range and 100 mV for fixed 2A

                                            range .

Integrator Output                     1 V = 2 AU.

Power consumption                 20 W.

Mains Voltage                        100/115/120/220/240V ±10%, 50/60 Hz.

Dimensions (LxWxH)              313 x 131 x 147 mm.

Weight                                   4.3 kg.